Purification of human adenosine deaminase for the preparation of a reference material

被引：6

作者：

Bota, A ^{[1
]}

Gella, FJ ^{[1
]}

Canalias, F ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, Unitat Bioquim Med, E-08193 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 737卷 / 1-2期

关键词：

purification; enzymes; adenosine deaminase;

D O I：

10.1016/S0378-4347(99)00359-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The goal was to optimise a purification procedure of adenosine deaminase from human erythrocytes for the preparation of a European Reference Material. Adenosine deaminase was purified from human erythrocytes with a specific activity of 4.46 mu ka/mg of protein and a catalytic concentration of 133 mu kat/l. The isolation and purification procedure involved ion-exchange chromatography (STREAMLINE(TM) DEAE), and two purine riboside affinity chromatographies. The purified enzyme exhibits a single band in SDS-PAGE with a molecular weight of 41600 g/mol, and three bands in PAGE, isoelectric focusing and two-dimensional electrophoresis with pI 4.7, 4.85 and 5.0. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：237 / 244

页数：8

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