Voltammetric determination of all DNA nucleotides

被引：283

作者：

Oliveira-Brett, AM ^{[1
]}

Piedade, JAP ^{[1
]}

Silva, LA ^{[1
]}

Diculescu, VC ^{[1
]}

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 332卷 / 02期

关键词：

guanine; adenine; thymine; cytosine; deoxynucleosides; deoxynucleotides; DNA; voltammetry; anodic oxidation;

D O I：

10.1016/j.ab.2004.06.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The voltammetric oxidation of all deoxyribonucleic acid (DNA) monophosphate nucleotides is investigated for the first time over a wide pH range by differential pulse voltammetry with a glassy carbon electrode. Experimental conditions such as the electrode size, supporting electrolyte composition, and pH were optimized to obtain the best peak potential separation and higher currents. This enabled the simultaneous voltammetric determination of all four DNA bases in equimolar mixtures and detection limits in the nanomolar range at physiological pH. It was also possible to detect for the first time the oxidation of each of the purine and pyrimidine nucleotides free in solution or as monomers in single-stranded DNA. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：321 / 329

页数：9

共 28 条

[1]

[Anonymous], 1998, ELECTROANAL

[2]

[Anonymous], ENCY ELECTROCHEMISTR

[3] Minor groove-binding architectural proteins: Structure, function, and DNA recognition [J].

Bewley, CA ;

Gronenborn, AM ;

Clore, GM .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1998, 27 :105-131

[4]

Brett AMO, 2000, ELECTROANAL, V12, P969, DOI 10.1002/1521-4109(200008)12:12<969::AID-ELAN969>3.0.CO

[5]

2-O

[6] Sonoelectrochemical determination of nucleosides and nucleotides [J].

Brett, AMO ;

Matysik, FM .

ULTRASONICS SONOCHEMISTRY, 1997, 4 (02) :125-126

[7] ON THE ADSORPTION AND ELECTROCHEMICAL OXIDATION OF DNA AT GLASSY-CARBON ELECTRODES [J].

BRETT, CMA ;

BRETT, AMO ;

SERRANO, SHP .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1994, 366 (1-2) :225-231

[8]

BRETT CMA, 1993, OXFORD SCI PUBLICATI

[9] Oxidative damage to DNA: formation, measurement and biochemical features [J].

Cadet, J ;

Douki, T ;

Gasparutto, D ;

Ravanat, JL .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 531 (1-2) :5-23

[10] ELECTROCHEMICAL OXIDATION-REDUCTION PATHS FOR PYRIMIDINE CYTOSINE PURINE AND ADENINE - CORRELATION AND APPLICATION [J].

DRYHURST, G ;

ELVING, PJ .

TALANTA, 1969, 16 (07) :855-&

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