Inhibitor fatty acid interactions with cytochrome P-450 BM3

被引：20

作者：

Macdonald, IDG

Smith, WE

Munro, AW

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,LANARK,SCOTLAND

[2] UNIV STRATHCLYDE,DEPT PURE & APPL CHEM,GLASGOW G1 1XL,LANARK,SCOTLAND

来源：

FEBS LETTERS | 1996年 / 396卷 / 2-3期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

cytochrome P450 BM3; SERRS spectroscopy; substrate interaction; heme environment; CD;

D O I：

10.1016/0014-5793(96)01095-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of fatty acid substrate (palmitate) and inhibitor (metyrapone: 2-methyl-1,2-di-3-pyridyl-1-propanone) with cytochrome P-450 BM3 was analysed by UV-visible and circular dichroism spectroscopy, and by surface-enhanced resonance Raman scattering (SERRS). While visible spectroscopy provides information on the relative affinities of these compounds, SERRS provides additional novel data indicating palmitate-induced structural changes in the haem environment. SERRS also demonstrates that binding of both palmitate and the large nitrogenous ligand metyrapone occurs simultaneously to P-450 BM3 - highlighting the usefulness of this technique in probing haemoprotein active sites.

引用

页码：196 / 200

页数：5

共 25 条

[1] RESONANCE RAMAN-SPECTRA OF OCTAETHYLPORPHYRINATO-NI(II) AND MESO-DEUTERATED AND N-15 SUBSTITUTED DERIVATIVES .2. NORMAL COORDINATE ANALYSIS [J].