Variability of laccase activity in the white-rot basidiomycete Pleurotus ostreatus

被引:22
作者
Baldrian, P [1 ]
Gabriel, J [1 ]
机构
[1] Acad Sci Czech Republic, Inst Microbiol, Lab Biochem Woodrotting Fungi, Prague 14220, Czech Republic
关键词
D O I
10.1007/BF02818695
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of laccase in liquid cultures of the white-rot fungus Pleurotus ostreatus was highly variable. During the first days of cultivation, the relative variability was as high as 80-100% and it decreased to 30% in the course of cultivation. The main source of variability was assumed to be the independent development of enzyme activity in individual cultures. Cultures with high laccase production showed also high production of the other ligninolytic enzyme - Mn-dependent peroxidase. The variability was probably due to the source of inoculum, deactivation of the enzyme in culture liquid and genetic variations among the cultures. Variability of laccase activities was lower during solid-state fermentation on wheat straw and during the growth in nonsterile soil.
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页码:385 / 390
页数:6
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