Determination of sulphur compounds in beer using headspace solid-phase microextraction and gas chromatographic analysis with pulsed flame photometric detection

被引:130
作者
Hill, PG
Smith, RM
机构
[1] Brauerei Beck & Co, D-28199 Bremen, Germany
[2] Loughborough Univ Technol, Dept Chem, Loughborough LE11 3TU, Leics, England
关键词
beer; food analysis; headspace analysis; extraction methods; detection; GC; sulphur compounds;
D O I
10.1016/S0021-9673(99)01307-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple and sensitive method for the analysis of volatile and semi-volatile sulphur compounds in beer at trace levels was developed using headspace solid-phase microextraction (SPME) and gas chromatography with pulsed flame photometric detection. Different SPME fibres were tested and a Carboxen-polydimethylsiloxane coated fibre was found to be the most appropriate. The adsorption and desorption conditions were optimised. The effect of ethanol concentration in the sample on the extraction of analytes was examined. A 60 m non-polar capillary column preceded by a 10 m length of a polar column was found to be capable of separating a wide range of C(1)-C(6) sulphur compounds. The pulsed flame photometric detector enabled increased sensitivity to be obtained over previous methods, such as dynamic headspace followed by conventional flame photometric detection or sulphur chemiluminescent detection, with high sulphur selectivity. Two sulphur compounds, 2-methyl-1-butanethiol and 3-methylthiophene, were identified in beer for the first time. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:203 / 213
页数:11
相关论文
共 62 条
[1]   PULSED FLAME PHOTOMETER DETECTOR FOR GAS-CHROMATOGRAPHY [J].
AMIRAV, A ;
JING, HW .
ANALYTICAL CHEMISTRY, 1995, 67 (18) :3305-3318
[2]   ORIGIN AND OCCURRENCE OF VOLATILE SULFUR-COMPOUNDS IN BRITISH ALES AND LAGERS [J].
ANDERSON, RJ ;
HOWARD, GA .
JOURNAL OF THE INSTITUTE OF BREWING, 1974, 80 (04) :357-370
[3]  
ANDERSON RJ, 1971, P EUROPEAN BREWERY C, P253
[4]  
ANNESS BJ, 1981, EUROPEAN BREWING CON, V7, P135
[5]  
[Anonymous], [No title captured]
[6]   AUTOMATION AND OPTIMIZATION OF SOLID-PHASE MICROEXTRACTION [J].
ARTHUR, CL ;
KILLAM, LM ;
BUCHHOLZ, KD ;
PAWLISZYN, J ;
BERG, JR .
ANALYTICAL CHEMISTRY, 1992, 64 (17) :1960-1966
[7]  
Atar E., 1991, Analytical Chemistry, V63, P2064
[8]  
Boelens Mans H., 1993, Perfumer and Flavorist, V18, P29
[9]  
BURMEISTER M S, 1992, Journal of the American Society of Brewing Chemists, V50, P53
[10]   PULSED-FLAME PHOTOMETER - A NOVEL GAS-CHROMATOGRAPHY DETECTOR [J].
CHESKIS, S ;
ATAR, E ;
AMIRAV, A .
ANALYTICAL CHEMISTRY, 1993, 65 (05) :539-555