Essential Role of the Glycosyltransferase Sxc/Ogt in Polycomb Repression

被引：255

作者：

Gambetta, Maria Cristina ^{[1
]}

Oktaba, Katarzyna ^{[1
]}

Mueller, Juerg ^{[1
]}

机构：

[1] EMBL, Gene Express Programme, D-69117 Heidelberg, Germany

来源：

SCIENCE | 2009年 / 325卷 / 5936期

关键词：

LINKED N-ACETYLGLUCOSAMINE; X-CHROMOSOME; GENE; GLYCOSYLATION; LOCALIZATION; PROTEINS; NUCLEAR; PATTERN;

D O I：

10.1126/science.1169727

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Polycomb group proteins are conserved transcriptional repressors that control animal and plant development. Here, we found that the Drosophila Polycomb group gene super sex combs (sxc) encodes Ogt, the highly conserved glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) to proteins in animals and plants. Genome-wide profiling in Drosophila revealed that GlcNAc-modified proteins are highly enriched at Polycomb response elements. Among different Polycomb group proteins, Polyhomeotic is glycosylated by Sxc/Ogt in vivo. sxc/Ogt-null mutants lacked O-linked GlcNAcylation and failed to maintain Polycomb transcriptional repression even though Polycomb group protein complexes were bound at their target sites. Polycomb repression appears to be a critical function of Sxc/Ogt in Drosophila and may be mediated by the glycosylation of Polyhomeotic.

引用

页码：93 / 96

页数：4

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