Use of the confocal microscope to determine polyomavirus recombinant capsid-like particle entry into mouse 3T6 cells

被引：9

作者：

An, K ^{[1
]}

Lovgren, TR ^{[1
]}

Tilley, MB ^{[1
]}

Consigli, RA ^{[1
]}

机构：

[1] Kansas State Univ, Div Biol, Sect Virol & Oncol, Manhattan, KS 66506 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 84卷 / 02期

基金：

美国国家航空航天局;

关键词：

confocal microscopy; polyomavirus; recombinant capsid-like particles; cell entry;

D O I：

10.1016/S0166-0934(99)00137-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like particles could be produced that were composed of the various structural proteins (VP1, VP1/2, VP1/3 and VP1/2 + VP3). Laser scanning confocal microscopy was used to determine if the various capsid-like particles could infect (enter) mouse 3T6 cells. Each of the various capsid-like particles was equally capable of cell entry as determined by indirect immunofluorescence confocal microscopy. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 159

页数：7

共 18 条

[1] Use of the baculovirus system to assemble polyomavirus capsid-like particles with different polyomavirus structural proteins: analysis of the recombinant assembled capsid-like particles
An, K
Gillock, ET
Sweat, JA
Reeves, WM
Consigli, RA
[J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 1009 - 1016
[2] Braun H, 1999, BIOTECHNOL APPL BIOC, V29, P31
[3] CHARACTERIZATION OF THE DNA-BINDING PROPERTIES OF POLYOMAVIRUS CAPSID PROTEINS
CHANG, DC
CAI, XY
CONSIGLI, RA
[J]. JOURNAL OF VIROLOGY, 1993, 67 (10) : 6327 - 6331
[4] THE USE OF ADDITIVE AND SUBTRACTIVE APPROACHES TO EXAMINE THE NUCLEAR-LOCALIZATION SEQUENCE OF THE POLYOMAVIRUS MAJOR CAPSID PROTEIN-VP1
CHANG, DC
HAYNES, JI
BRADY, JN
CONSIGLI, RA
[J]. VIROLOGY, 1992, 189 (02) : 821 - 827
[5] EXPRESSION OF THE POLYOMAVIRUS VP2 AND VP3 PROTEINS IN INSECT CELLS - COEXPRESSION WITH THE MAJOR CAPSID PROTEIN VP1 ALTERS VP2/VP3 SUBCELLULAR-LOCALIZATION
DELOS, SE
MONTROSS, L
MORELAND, RB
GARCEA, RL
[J]. VIROLOGY, 1993, 194 (01) : 393 - 398
[6] FORSTOVA J, 1993, J VIROL, V67, P1405
[7] POLYOMA-VIRUS PSEUDOCAPSIDS AS EFFICIENT CARRIERS OF HETEROLOGOUS DNA INTO MAMMALIAN-CELLS
FORSTOVA, J
KRAUZEWICZ, N
SANDIG, V
ELLIOTT, J
PALKOVA, Z
STRAUSS, M
GRIFFIN, BE
[J]. HUMAN GENE THERAPY, 1995, 6 (03) : 297 - 306
[8] Truncation of the nuclear localization signal of polyomavirus VP1 results in a loss of DNA packaging when expressed in the baculovirus system
Gillock, ET
An, K
Consigli, RA
[J]. VIRUS RESEARCH, 1998, 58 (1-2) : 149 - 160
[9] Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system
Gillock, ET
Rottinghaus, S
Chang, D
Cai, X
Smiley, SA
An, K
Consigli, RA
[J]. JOURNAL OF VIROLOGY, 1997, 71 (04) : 2857 - 2865
[10] EARLY EVENTS IN POLYOMAVIRUS INFECTION - FUSION OF MONOPINOCYTOTIC VESICLES CONTAINING VIRIONS WITH MOUSE KIDNEY-CELL NUCLEI
GRIFFITH, GR
MARRIOTT, SJ
RINTOUL, DA
CONSIGLI, RA
[J]. VIRUS RESEARCH, 1988, 10 (01) : 41 - 51

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