An evaporation-based microfluidic sample concentration method

被引：91

作者：

Walker, GM ^{[1
]}

Beebe, DJ ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA

来源：

LAB ON A CHIP | 2002年 / 2卷 / 02期

关键词：

D O I：

10.1039/b202473j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method for sample concentration within microfluidic devices using evaporation-induced flow. Evaporation-induced flow is easy to incorporate into microfluidic designs and can be used to concentrate a wide variety of molecules. The practicality of this method was demonstrated with 0.2 mum fluorescent spheres and FITC- labeled BSA. Thirty two percent of the 0.6 muL fluorescent sphere suspension was concentrated into a well within a microfluidic device. In the same amount of time, 93% of the 0.6 muL FITC- labeled BSA solution was concentrated.

引用

页码：57 / 61

页数：5

共 16 条

[1] Bird R.B., 2006, TRANSPORT PHENOMENA, Vsecond, DOI 10.1002/aic.690070245
[2] Rapid, automated nucleic acid probe assays using silicon microstructures for nucleic acid concentration
Christel, LA
Petersen, K
McMillan, W
Northrup, MA
[J]. JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1999, 121 (01): : 22 - 27
[3] Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays
Dodge, A
Fluri, K
Verpoorte, E
de Rooij, NF
[J]. ANALYTICAL CHEMISTRY, 2001, 73 (14) : 3400 - 3409
[4] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)
Duffy, DC
McDonald, JC
Schueller, OJA
Whitesides, GM
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4974 - 4984
[5] An evaporation-based disposable micropump concept for continuous monitoring applications
Effenhauser, CS
Harttig, H
Krämer, P
[J]. BIOMEDICAL MICRODEVICES, 2002, 4 (01) : 27 - 32
[6] Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer
Jo, BH
Van Lerberghe, LM
Motsegood, KM
Beebe, DJ
[J]. JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2000, 9 (01) : 76 - 81
[7] Kutter JP, 2000, J MICROCOLUMN SEP, V12, P93, DOI 10.1002/(SICI)1520-667X(2000)12:2<93::AID-MCS5>3.0.CO
[8] 2-P
[9] McAdams W. H., 1954, HEAT TRANSMISSION
[10] MENG A, 1999, TRANSDUCERS, P876

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