Design and application of a biosensor for monitoring toxicity of compounds to eukaryotes

被引:51
作者
Hollis, RP
Killham, K
Glover, LA
机构
[1] Univ Aberdeen, Dept Mol & Cell Biol, Inst Med Sci, Aberdeen AB25 2ZD, Scotland
[2] Univ Aberdeen, Dept Plant & Soil Sci, Aberdeen AB24 3UU, Scotland
关键词
D O I
10.1128/AEM.66.4.1676-1679.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Here we describe an alternative approach to currently used cytotoxicity analyses through applying eukaryotic microbial biosensors. The yeast Saccharomyces cererisiae was genetically modified to express firefly luciferase, generating a bioluminescent yeast strain, The presence of any toxic chemical that interfered with the cells' metabolism resulted in a quantitative decrease in bioluminescence. In this study, it was demonstrated that the Luminescent yeast strain senses chemicals known to be toxic to eukaryotes in samples assessed as nontoxic by prokaryotic biosensors. As the cell wall and adaptive mechanisms of S. cerevisiae cells enhance stability and protect from extremes of pH, solvent exposure, and osmotic shock, these inherent properties were exploited to generate a biosensor that should detect a wide range of both organic and inorganic toxins under extreme conditions.
引用
收藏
页码:1676 / 1679
页数:4
相关论文
共 20 条
[1]   Toxicity order of cholanic acids using an immobilised cell biosensor [J].
Campanella, L ;
Favero, G ;
Mastrofini, D ;
Tomassetti, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 14 (8-10) :1007-1013
[2]   EFFECTS OF A HETEROGENOUS SET OF XENOBIOTICS ON GROWTH AND PLASMA-MEMBRANES OF MAMMALIAN AND FUNGAL CELL-CULTURES [J].
CASCORBI, I ;
BITTRICH, H ;
RICKLINKAT, J ;
VOSS, W ;
SEYFARTH, A ;
FORET, M .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 1993, 26 (01) :113-126
[3]  
DAMORE T, 1990, CRIT REV BIOTECHNOL, V9, P287, DOI 10.3109/07388558909036740
[4]   mRNA translation in yeast during entry into stationary phase [J].
Dickson, LM ;
Brown, AJP .
MOLECULAR AND GENERAL GENETICS, 1998, 259 (03) :282-293
[5]   IMPROVED METHOD FOR HIGH-EFFICIENCY TRANSFORMATION OF INTACT YEAST-CELLS [J].
GIETZ, D ;
STJEAN, A ;
WOODS, RA ;
SCHIESTL, RH .
NUCLEIC ACIDS RESEARCH, 1992, 20 (06) :1425-1425
[6]  
Guldfeldt LU, 1998, APPL ENVIRON MICROB, V64, P530
[7]   RAPID TOXICITY TESTING BASED ON YEAST RESPIRATORY ACTIVITY [J].
HAUBENSTRICKER, ME ;
MEIER, PG ;
MANCY, KH ;
BRABEC, MJ .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1990, 44 (05) :669-674
[8]  
Henderson G, 1989, Biol Met, V2, P83, DOI 10.1007/BF01129205
[9]   Microbial cyanide sensor for monitoring river water [J].
Ikebukuro, K ;
Miyata, A ;
Cho, SJ ;
Nomura, Y ;
Chang, SM ;
Yamauchi, Y ;
Hasebe, Y ;
Uchiyama, S ;
Karube, I .
JOURNAL OF BIOTECHNOLOGY, 1996, 48 (1-2) :73-80
[10]  
KOCH HP, 1993, METHOD FIND EXP CLIN, V15, P141