共 94 条
Identification of the Neuroblastoma-amplified Gene Product as a Component of the Syntaxin 18 Complex Implicated in Golgi-to-Endoplasmic Reticulum Retrograde Transport
被引:101
作者:
Aoki, Takehiro
[1
]
Ichimura, Sarah
[1
]
Itoh, Ayano
[1
]
Kuramoto, Mami
[1
]
Shinkawa, Takashi
[2
]
Isobe, Toshiaki
[2
]
Tagaya, Mitsuo
[1
]
机构:
[1] Tokyo Univ Pharm & Life Sci, Sch Life Sci, Tokyo 1920392, Japan
[2] Tokyo Metropolitan Univ, Grad Sch Sci, Dept Chem, Tokyo 1920397, Japan
关键词:
ER-ASSOCIATED DEGRADATION;
EARLY SECRETORY PATHWAY;
MEMBRANE-FUSION;
SNARE COMPLEX;
INTERMEDIATE COMPARTMENT;
SACCHAROMYCES-CEREVISIAE;
RAD50-INTERACTING PROTEIN;
VESICLE TRAFFICKING;
RETRIEVAL-SYSTEM;
DYNEIN FUNCTION;
D O I:
10.1091/mbc.E08-11-1104
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Syntaxin 18, a soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) protein implicated in endoplasmic reticulum (ER) membrane fusion, forms a complex with other SNAREs (BNIP1, p31, and Sec22b) and several peripheral membrane components (Sly1, ZW10, and RINT-1). In the present study, we showed that a peripheral membrane protein encoded by the neuroblastoma-amplified gene (NAG) is a subunit of the syntaxin 18 complex. NAG encodes a protein of 2371 amino acids, which exhibits weak similarity to yeast Dsl3p/Sec39p, an 82-kDa component of the complex containing the yeast syntaxin 18 orthologue Ufe1p. Under conditions favoring SNARE complex disassembly, NAG was released from syntaxin 18 but remained in a p31-ZW10-RINT-1 subcomplex. Binding studies showed that the extreme N-terminal region of p31 is responsible for the interaction with NAG and that the N- and the C-terminal regions of NAG interact with p31 and ZW10-RINT-1, respectively. Knockdown of NAG resulted in a reduction in the expression of p31, confirming their intimate relationship. NAG depletion did not substantially affect Golgi morphology and protein export from the ER, but it caused redistribution of Golgi recycling proteins accompanied by a defect in protein glycosylation. These results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport.
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页码:2639 / 2649
页数:11
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