Sum-frequency generation spectroscopy applied to model biosensors systems

被引：22

作者：

Dreesen, L

Sartenaer, Y

Humbert, C

Mani, AA

Lemaire, JJ

Méthivier, C

Pradier, CM

Thiry, PA

Peremans, A

机构：

[1] Univ Namur, Lab Spect Mol Surface, B-5000 Namur, Belgium

[2] Ecole Natl Super Chim Paris, CNRS, UMR 7045, Lab Physicochim Surface, F-75231 Paris 05, France

来源：

THIN SOLID FILMS | 2004年 / 464卷

关键词：

biosensors; avidin; biotin; sum-frequency generation;

D O I：

10.1016/j.tsf.2004.06.108

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：373 / 378

页数：6

共 26 条

[1] Studies of polymer surfaces by sum frequency generation vibrational spectroscopy [J].