Pathology and ultrastructure of Hz-2V infection in the agonadal female corn earworm, Helicoverpa zea

被引:9
作者
Rallis, CP
Burand, JP
机构
[1] Univ Massachusetts, Dept Entomol, Amherst, MA 01003 USA
[2] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA
关键词
accessory glands; bursa copulatrix; copulation; culticular lining; gonad-specific virus; Helicoverpa zea; Hz-2V; insect virus; ovaries; oviduct; sex pheromone; sexual receptivity; ultrastructure; waxy plug;
D O I
10.1016/S0022-2011(02)00113-1
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
The pathology and ultrastructure of the reproductive tract of Hz-2V-infected female corn earworm moths, Helicoverpa zea, were studied. The identity of malformed reproductive tissues found in virus-infected moths was determined by examining these tissues in moths that were infected with the virus at different life stages. Malformation of reproductive tissues in the progeny of virus-infected female moths was first observed by 3 days post-pupation (dpp), indicating that virus replication had altered the differentiation of these tissues very early on in their development. The ultrastructure of the grossly malformed agonadal reproductive tissues from insects aged 3-10dpp revealed the absence of the cuticular lining found in the oviducts of normal moths, and the proliferation of epithelial cells in these infected oviduct tissues. In addition, large quantities of virus were found aggregated into a large mass in the lumen of the malformed cervix bursa of 10 dpp agonadal female pharate adult moths. Prior to eclosion, the virus in the cervix bursa was observed separated into spherical masses, which are thought to exude through the ductus bursa and collect over the vulva, forming a viral "waxy plug" that is likely to play an important role in virus transmission. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:33 / 44
页数:12
相关论文
共 8 条
[1]   Replication of a gonad-specific insect virus in TN-368 cells in culture [J].
Burand, JP ;
Lu, H .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1997, 70 (02) :88-95
[2]  
CALLAHAN PHILIP S., 1958, ANN ENT SOC AMER, V51, P413
[3]  
CALLAHAN PHILIP S., 1963, ANN ENTOMOL SOC AMER, V56, P535
[4]   Oviposition day effect on incidence of agonadal progeny of Helicoverpa zea (Lepidoptera: Noctuidae) infected with a virus [J].
Hamm, JJ ;
Carpenter, JE ;
Styer, EL .
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1996, 89 (02) :266-275
[5]   Replication of the gonad-specific virus Hz-2V in Ld652Y cells mimics replication in vivo [J].
Lu, H ;
Burand, JP .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2001, 77 (01) :44-50
[6]   GONAD-SPECIFIC VIRUS OF CORN-EARWORM [J].
RAINA, AK ;
ADAMS, JR .
NATURE, 1995, 374 (6525) :770-770
[7]   Further characterization of the gonad-specific virus of corn earworm, Helicoverpa zea [J].
Raina, AK ;
Adams, JR ;
Lupiani, B ;
Lynn, DE ;
Kim, WJ ;
Burand, JP ;
Dougherty, EM .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2000, 76 (01) :6-12
[8]   Pathology and ultrastructure of the insect virus, Hz-2V, infecting agonadal male corn earworms, Helicoverpa zea [J].
Rallis, CP ;
Burand, JP .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2002, 80 (02) :81-89