Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli

被引：2

作者：

Kobori, T

Lee, WC

Akagi, T

Sasaki, H

Zenno, S

Saigo, K

Tanokura, M

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

[3] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[4] Univ Tokyo, Dept Biochem & Biophys, Grad Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999008422

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NADPH:nitrocompound oxiddoreductase from Escherichia coli, NfsA, has been crystallized in the presence of FMN by the vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belonged to the triclinic space group PI with cell dimensions, a = 52.2, b = 52.7, c = 53.3 Angstrom, alpha = 75.1, beta = 60.1, gamma = 60.5 degrees. The crystals are expected to contain two NfsA molecules per asymmetric unit. The crystals diffracted X-rays to at least 2.3 Angstrom resolution and are appropriate for structural analysis at high resolution.

引用

页码：1901 / 1902

页数：2

共 18 条

[1] THE BIOACTIVATION OF 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB1954) .1. PURIFICATION AND PROPERTIES OF A NITROREDUCTASE ENZYME FROM ESCHERICHIA-COLI - A POTENTIAL ENZYME FOR ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) [J].