Centrosomal anchoring of the protein kinase CK1δ mediated by attachment to the large, coiled-coil scaffolding protein CG-NAP/AKAP450

被引:90
作者
Sillibourne, JE
Milne, DM
Takahashi, M
Ono, Y
Meek, DW [1 ]
机构
[1] Univ Dundee, Biomed Res Ctr, Ninewells Hosp, Dundee DD1 9SY, Scotland
[2] Univ Dundee, Sch Med, Dundee DD1 9SY, Scotland
[3] Kobe Univ, Dept Biol, Biosignal Res Ctr, Nada Ku, Kobe, Hyogo 6578501, Japan
基金
英国医学研究理事会;
关键词
protein kinase; CK1; AKAP; anchor; centrosome;
D O I
10.1016/S0022-2836(02)00857-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinase CK1 (formerly termed casein kinase 1) is ubiquitous in eukaryotic cells and comprises a family of as many as 14 isoforms (including splice variants) in mammalian cells. Mammalian CK1delta and CK1epsilon, which are highly related to each other, are enriched at the centrosomes in interphase cells and at the spindle during mitosis. In the present study we have isolated, using the yeast two-hybrid system, a 182 amino acid residue fragment of the centrosomal and golgi N-kinase anchoring protein (CG-NAP, also known as AKAP450), which specifically interacts with CK1delta and CK1epsilon, but not with other CK1 isoforms. The 182 amino acid residue CG-NAP fragment, or full length CG-NAP, co-immunoprecipitates with CK1delta and CK1epsilon from mammalian cells. Consistent with this association, endogenous CG-NAP/AKAP450 and CK1delta co-localize in cells. Moreover, when expressed in the presence of CK1delta the 182 amino acid residue CG-NAP fragment adopts the same sub-cellular localization as CK1delta. Strikingly, attachment of the CG-NAP fragment to the plasma membrane is sufficient to re-localize a significant level of CK1delta to the membrane. These findings support a model in which sub-cellular localization of CK1delta/epsilon molecules at the centrosome is mediated, at least in part, through the action of CG-NAP/AKAP450 and provide a potential mechanism by which the contribution to cell cycle progression by CK1delta/ epsilon may be regulated. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:785 / 797
页数:13
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