Electron transfer from the nitrogenase iron protein to the [8Fe-(7/8)S] clusters of the molybdenum-iron protein

The reduction of substrates catalyzed by nitrogenase requires electron transfer between the iron (Fe) protein and the molybdenum-iron (MoFe) protein in a reaction that is coupled to the hydrolysis of MgATP. The [4Fe-4S] cluster of the Fe protein transfers one electron ultimately to the M-clusters (FeMoco) of the MoFe protein for substrate reduction, with the P-clusters ([8Fe-(7/8)S]) of the MoFe protein as proposed electron transfer intermediates. This work presents direct EPR evidence for primary electron transfer from the [4Fe-4S] cluster of the Fe protein to the P-clusters of the MoFe protein in a reaction that requires the MgATP-bound state of the Fe protein. An oxidized state of the MoFe protein was prepared in which the P-clusters were oxidized by 2 equiv of electrons to the P2+ state. In this oxidation state, the M-clusters (S = 3/2) and the P2+-clusters (S greater than or equal to 3) are paramagnetic and can be observed by perpendicular and parallel mode EPR, providing the opportunity to follow electron transfer from the Fe protein to either cluster type in the MoFe protein. Electron transfer from the reduced [4Fe-4S](1+) cluster of two different Fe proteins to the P2+ clusters of the MoFe protein was observed by the disappearance of the [4Fe-4S](1+) cluster EPR signal and the conversion of the MoFe protein P-clusters from the P2+ to the P1+ oxidation state. In the first case, stoichiometric quantities of the wild-type Fe protein transferred one electron to the P-clusters only in the presence of MgATP. MgADP would not support this electron transfer reaction. In the second case, an altered Fe protein (L127 Delta) that is in a conformation resembling the MgATP-bound state was found to transfer an electron to the P-clusters in the absence of MgATP. These results suggest that the first electron transferred from the Fe protein goes to the P-cluster and that the MgATP-bound protein conformation of the Fe protein, not MgATP hydrolysis, is required for this electron transfer reaction.