ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure

被引：10

作者：

Greenway, DLA ^{[1
]}

England, RR ^{[1
]}

机构：

[1] Univ Cent Lancashire, Dept Biol Sci, Preston PR1 2HE, Lancs, England

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1999.00615.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of a commonly used biocide, 1,2- benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads.

引用

页码：298 / 302

页数：5

共 26 条

[1] OCCURRENCE OF RESISTANCE TO ANTIBIOTICS AND METALS AND OF PLASMIDS IN BACTERIAL STRAINS ISOLATED FROM MARINE ENVIRONMENTS [J].

AVILES, M ;

CODINA, JC ;

PEREZGARCIA, A ;

CAZORLA, F ;

ROMERO, P ;

DEVICENTE, A .

WATER SCIENCE AND TECHNOLOGY, 1993, 27 (3-4) :475-478

[2] THE INFLUENCE OF ENVIRONMENT ON ENVELOPE PROPERTIES AFFECTING SURVIVAL OF BACTERIA IN INFECTIONS [J].

BROWN, MRW ;

WILLIAMS, P .

ANNUAL REVIEW OF MICROBIOLOGY, 1985, 39 :527-556

[3]

Cashel M., 1996, ESCHERICHIA COLI SAL, V1, P1458

[4] EVALUATION OF THE BACTERICIDAL ACTIVITY OF BETA-LACTAM ANTIBIOTICS ON SLOWLY GROWING BACTERIA CULTURED IN THE CHEMOSTAT [J].

COZENS, RM ;

TUOMANEN, E ;

TOSCH, W ;

ZAK, O ;

SUTER, J ;

TOMASZ, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1986, 29 (05) :797-802

[5]

FULLER SJ, 1986, THESIS U NOTTINGHAM

[6] INFLUENCE OF GROWTH-RATE ON SUSCEPTIBILITY TO ANTIMICROBIAL AGENTS - BIOFILMS, CELL-CYCLE, DORMANCY, AND STRINGENT RESPONSE [J].

GILBERT, P ;

COLLIER, PJ ;

BROWN, MRW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (10) :1865-1868

[7] ADHESIVE COLONIZATION OF BIOMATERIALS AND ANTIBIOTIC-RESISTANCE [J].

GRISTINA, AG ;

HOBGOOD, CD ;

WEBB, LX ;

MYRVIK, QN .

BIOMATERIALS, 1987, 8 (06) :423-426

[8] Accumulation of ppGpp in symbiotic and free-living nitrogen-fixing bacteria following amino acid starvation [J].

Howorth, SM ;

England, RR .

ARCHIVES OF MICROBIOLOGY, 1999, 171 (02) :131-134

[9] Guanosine 5'-diphosphate 3'-diphosphate (ppGpp), guanosine 5'-diphosphate 3'-monophosphate (ppGp) and antibiotic production in Streptomyces clavuligerus [J].

Jones, C ;

Thompson, A ;

England, R .

MICROBIOLOGY-SGM, 1996, 142 :1789-1795

[10] PPGPP CONCENTRATION, GROWTH WITHOUT PBP2 ACTIVITY, AND GROWTH-RATE CONTROL IN ESCHERICHIA-COLI [J].

JOSELEAUPETIT, D ;

THEVENET, D ;

DARI, R .

MOLECULAR MICROBIOLOGY, 1994, 13 (05) :911-917

← 1 2 3 →