A protocol for repetitive somatic embryogenesis from mature peanut epicotyls

被引：47

作者：

Little, EL ^{[1
]}

Magbanua, ZV ^{[1
]}

Parrott, WA ^{[1
]}

机构：

[1] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 04期

关键词：

picloram; centrophenoxine; auxin; Arachis hypogaea L; somatic embryogenesis;

D O I：

10.1007/s002990050739

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effects of 11 different auxins and one cytokinin-like compound were tested at four concentrations for their ability to induce primary and repetitive somatic embryos from mature, dry peanut (Arachis hypogaea L.) epicotyls of genotype AT120. Treatment with picloram and centrophenoxine at 83.0 and 124.4 mu M resulted in the greatest number of embryos per explant and the highest percentage of explants responding. In a follow-up experiment, picloram, centrophenoxine, and dicamba were tested at 83.0 and 124.4 mu M on four peanut genotypes (AT120, 59-4144, GK7, and VC1). Picloram and centrophenoxine induced similar numbers of globular-stage and total embryos from each genotype, while dicamba was less effective. Similar results were observed with percentage of responding axes. Genotypes AT120 and VC1 yielded more clusters of repetitive embryos than GK7 and 59-4144. After 5 months, embryos derived from repetitive embryogenic cultures were converted into mature plants.

引用

页码：351 / 357

页数：7

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