High resolution electrophysiological activity imaging of in-vitro neuronal networks

被引：8

作者：

Berdondini, L ^{[1
]}

Overstolz, T ^{[1
]}

de Rooij, NF ^{[1
]}

Koudelka-Hep, M ^{[1
]}

Martinoia, S ^{[1
]}

Seitz, P ^{[1
]}

Wäny, M ^{[1
]}

Blanc, N ^{[1
]}

机构：

[1] Univ Neuchatel, IMT, CH-2000 Neuchatel, Switzerland

来源：

2ND ANNUAL INTERNATIONAL IEEE-EMBS SPECIAL TOPIC CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE & BIOLOGY, PROCEEDINGS | 2002年

关键词：

MEA; high density array; in-vitro neuronal networks; extracellular recording;

D O I：

10.1109/MMB.2002.1002322

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A prototype of a high density microelectrode array (MEA) for in-vitro recording of the electrophysiological activity of neuronal cell cultures has been developed. Based on standard CMOS technologies and dedicated post processing steps, it integrates 64x64 gold electrodes of 20x20 mum(2), organized in a pixel array on a surface of 2.56x2.56 mm(2). Each pixel has a dimension of 40x40 mum(2), resulting in an electrode separation of 40 mum. The on-chip integrated logic allows to randomly select each pixel individually, enabling the read out at high sampling rate of the whole active area or of limited zones of high neuronal activity. Additionally, each pixel integrates an amplifier circuit for local signal amplification with programmable gain and filtering. Simulation results and a first electrical characterisation in phosphate solution are presented in this paper.

引用

页码：241 / 244

页数：4

共 18 条

[1] Microelectronic sensor system for microphysiological application on living cells [J].