Cmk2, a novel serine/threonine kinase in fission yeast

被引：11

作者：

Alemany, V ^{[1
]}

Sanchez-Piris, M ^{[1
]}

Bachs, O ^{[1
]}

Aligue, R ^{[1
]}

机构：

[1] Univ Barcelona, Fac Med, Inst Invest Biomed August Pi I Sunyer, Dept Cellular Biol, E-08036 Barcelona, Spain

来源：

FEBS LETTERS | 2002年 / 524卷 / 1-3期

关键词：

fission yeast; calmodulin-dependent kinase; mitosis; cell growth; actin; replication checkpoint;

D O I：

10.1016/S0014-5793(02)03006-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cmk2 gene of Schizosaccharonryces pombe encodes a 504 amino acid protein kinase with sequence homology with the calmodulin-dependent protein kinase family. The cmk(2+) gene is not essential for cell viability but overexpression of cmk(2+) blocks the cell cycle at G2 phase and this inhibition is cdc2-dependent. The Cmk2 is a cytoplasmic protein expressed in a cell cycle-dependent manner, peaking at the GUS boundary. Overexpression of Cmk2 suppresses fission yeast DNA replication checkpoint defects but not DNA damage checkpoint defects, suggesting that the G2 cell cycle arrest mediated by high levels of Cmk2 provides sufficient time to correct DNA replication alterations. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：79 / 86

页数：8

共 26 条

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