BEAD-BASED MICROFLUIDIC PLATFORM INTEGRATED WITH OPTICAL DETECTION DEVICES FOR RAPID DETECTION OF GENETIC DELETION FROM SALIVA

被引：1

作者：

Lien, Kang-Yi ^{[1
]}

Liu, Chien-Ju ^{[3
]}

Kuo, Pao-Lin ^{[2
]}

Lee, Gwo-Bin ^{[1
,3
,4
]}

机构：

[1] Natl Cheng Kung Univ, Inst Nanotechnol & Microsyst Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Dept Obstet & Gynecol, Tainan, Taiwan

[3] Natl Cheng Kung Univ, Dept Obstet & Gynecol, Tainan, Taiwan

[4] Ind Technol Res Inst, Med Elect & Device Technol Ctr, Hsinchu, Taiwan

来源：

IEEE 22ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2009) | 2009年

关键词：

QUALITY GENOMIC DNA; EXTRACTION; SYSTEMS; FLUID;

D O I：

10.1109/MEMSYS.2009.4805388

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.

引用

页码：340 / 343

页数：4

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