Efficient expression of foreign proteins in roots from tobravirus vectors

被引：80

作者：

MacFarlane, SA ^{[1
]}

Popovich, AH ^{[1
]}

机构：

[1] Scottish Crop Res Inst, Dept Virol, Dundee DD2 5DA, Scotland

来源：

VIROLOGY | 2000年 / 267卷 / 01期

关键词：

plant virus vectors; root delivery; plant biotechnology; tobraviruses;

D O I：

10.1006/viro.1999.0098

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viral vectors were constructed from infectious cDNA clones of each of the three tobraviruses, tobacco rattle virus (TRV), pea early-browning virus (PEBV), and pepper ringspot virus (PepRSV). RNA2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express green fluorescent protein (GFP) when inoculated to plants. The tobravirus expression vectors have a wide host range and were able to express GFP in, for example, Nicotiana species, tomato, pea, arabidopsis, and sugar beet. The TRV vector was able to invade and express GFP very efficiently in roots, whereas the widely used PVX vector was not. (C) 2000 Academic Press.

引用

页码：29 / 35

页数：7

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