Amphiphilic block copolymers based on poly (2-acryloyloxyethyl phosphorylcholine) prepared via RAFT polymerisation as biocompatible nanocontainers

被引:109
作者
Stenzel, MH [1 ]
Barner-Kowollik, C
Davis, TP
Dalton, HM
机构
[1] Univ New S Wales, Sch Chem Engn & Ind Chem, Ctr Adv Macromol Design, Sydney, NSW 2031, Australia
[2] Univ New S Wales, Sch Biotechnol & Biomol Sci, Ctr Adv Macromol Design, Sydney, NSW 2031, Australia
关键词
biocompatibility; diblock copolymers; micelles; poly(2-acryloyloxyethyl phosphorylcholine); RAFT polymerisation;
D O I
10.1002/mabi.200300113
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Amphiphilic block copolymers composed of poly(butyl acrylate) and poly(2-acryloyloxyethyl phosphorylcholine) have been prepared using reversible addition fragmentation transfer (RAFT) polymerisation. The conversion of the polymerisation was determined using online FT NIR spectroscopy. NMR spectroscopy was used not only to support the results obtained from FT NIR spectroscopy but also prove the formation of micelles. Due to the strong aggregation tendency of these block copolymers and the resulting difficulties concerning the molecular weight analysis test experiments were carried out replacing poly(2-acryloyloxyethyl phosphorylcholine) with poly(2-hydroxyethyl acrylate). Micelle size and the aggregation behaviour were investigated using dynamic light scattering. The sizes of the nanocontainers obtained were found to be influenced by the block length as well as the solvent leading to micelles in the range between 40 and 160 nm. The toxicity of the RAFT agent used was then analysed by cell growth inhibition tests.
引用
收藏
页码:445 / 453
页数:9
相关论文
共 43 条
[1]   Modeling the reversible addition-fragmentation chain transfer process in cumyl dithiobenzoate-mediated styrene homopolymerizations: Assessing rate coefficients for the addition-fragmentation equilibrium [J].
Barner-Kowollik, C ;
Quinn, JF ;
Morsley, DR ;
Davis, TP .
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2001, 39 (09) :1353-1365
[2]   RAFTing down under: Tales of missing radicals, fancy architectures, and mysterious holes [J].
Barner-Kowollik, C ;
Davis, TP ;
Heuts, JPA ;
Stenzel, MH ;
Vana, P ;
Whittaker, M .
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2003, 41 (03) :365-375
[3]   Effect of sodium dodecyl sulfate on the morphology of polystyrene-b-poly(acrylic acid) aggregates in dioxane-water mixtures [J].
Burke, SE ;
Eisenberg, A .
LANGMUIR, 2001, 17 (26) :8341-8347
[4]   Unusual aggregation behavior of a novel tertiary amine methacrylate-based diblock copolymer: Formation of micelles and reverse micelles in aqueous solution [J].
Butun, V ;
Billingham, NC ;
Armes, SP .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (45) :11818-11819
[5]  
CAMPBELL EJ, 1994, ASAIO J, V40, P853
[6]   Polymer vesicles [J].
Discher, DE ;
Eisenberg, A .
SCIENCE, 2002, 297 (5583) :967-973
[7]   Sulfobetaine-containing diblock and triblock copolymers via reversible addition-fragmentation chain transfer polymerization in aqueous media [J].
Donovan, MS ;
Lowe, AB ;
Sanford, TA ;
McCormick, CL .
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2003, 41 (09) :1262-1281
[8]   Micellization of strongly segregated block copolymers [J].
Forster, S ;
Zisenis, M ;
Wenz, E ;
Antonietti, M .
JOURNAL OF CHEMICAL PHYSICS, 1996, 104 (24) :9956-9970
[9]   Dilute solution behaviour of carboxylatobetaine end-capped polyisoprenes [J].
Gnambodoe, M ;
Hamaide, T ;
Revillon, A .
POLYMER, 1996, 37 (04) :603-613
[10]   New polymer synthesis by nitroxide mediated living radical polymerizations [J].
Hawker, CJ ;
Bosman, AW ;
Harth, E .
CHEMICAL REVIEWS, 2001, 101 (12) :3661-3688