Lentiviral manipulation of gene expression in human adult and embryonic stem cells

被引:31
作者
Clements, Mark O.
Godfrey, Andrew
Crossley, Joanne
Wilson, Sam J.
Takeuchi, Yasu
Boshoff, Chris
机构
[1] UCL, Wolfson Inst Biomed Res, London WC1E 6BT, England
[2] Windeyer Inst Med Sci, London, England
来源
TISSUE ENGINEERING | 2006年 / 12卷 / 07期
基金
英国惠康基金; 英国医学研究理事会;
关键词
D O I
10.1089/ten.2006.12.1741
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery. To achieve this goal, genetic tools need to be optimized and developed for controlling and manipulating stem cells ex vivo. Here we describe a lentiviral delivery system capable of high infection rates in human mesenchymal and embryonic stem cells. The lentiviral backbone was modified to express mono- and bi-cistronic transgenes and was also used to deliver short hairpin ribonucleic acid for specific silencing of gene expression in human stem cells. We show that lentiviral transduction can be used to alter gene expression without altering the genes' ability to differentiate in vitro. These vectors will enable rapid analysis of gene function in stem cells and permit the generation of knock-in/knock-out models of human disease in the rapidly developing field of gene therapy.
引用
收藏
页码:1741 / 1751
页数:11
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