Mannose selection system used for cucumber transformation

被引:60
作者
He, Zhengquan
Duan, ZhenZhen
Liang, Wei
Chen, Faju
Yao, Wei
Liang, Hongwei
Yue, Chaoyin
Sun, Zongxiu
Chen, Fan
Dai, Jianwu [1 ]
机构
[1] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[2] China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Peoples R China
[3] China Natl Rice Res Inst, Natl Key Lab Rice Biol, Hangzhou 310006, Peoples R China
关键词
Agrobacterium-mediated transformation; Cucumis sativus L; phosphomannose-isomerase; positive selection;
D O I
10.1007/s00299-006-0156-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.
引用
收藏
页码:953 / 958
页数:6
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