Nafion-methylene blue functional membrane and its application in chemical and biosensing

被引：24

作者：

Hong, Jun ^{[1
]}

Ghourchian, Hedayatollah ^{[1
]}

Rezaei-Zarchi, Saeed ^{[1
]}

Moosavi-Movahedi, Ali Akbar ^{[1
]}

Ahmadian, Shahin ^{[1
]}

Saboury, Ali Akbar ^{[1
]}

机构：

[1] Univ Tehran, Inst Biochem & Biophys, Tehran 1981813563, Iran

来源：

ANALYTICAL LETTERS | 2007年 / 40卷 / 03期

基金：

美国国家科学基金会;

关键词：

methylene blue; Nafion; functional membrane; biosensor; HRP; hydrogen peroxide;

D O I：

10.1080/00032710601017664

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A functional membrane composed of nanoparticles of methylene blue and Nafion perfluorosulfonated ion-exchange resin ( Nafion (R)) was constructed and characterized by the methods of scanning electron microscope, transmission electron microscope, UV-vis, and Fourier transform infrared. The membrane at glassy carbon electrode was directly applicable for determining H2O2 concentration. Furthermore, horseradish peroxidase was immobilized on the membrane, which showed a quasi-reversible electrochemical behavior with a formal potential of 2296 mV. The resulting biosensor exhibited a good stability and higher sensitivity to H2O2 relative to the membrane without horseradish perioxidase. The linear range of this biosensor for H2O2 determination was from 2 x 10(-5) to 7 x 10(-3) M, with detection limit of 7 x 10(-6) M.

引用

页码：483 / 496

页数：14

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