Overexpression of a member of the pentraxin family (PTX3) in human soft tissue liposarcoma

被引：57

作者：

Willeke, F.

Assad, A.

Findeisen, P.

Schromm, E.

Grobholz, R.

von Gerstenbergk, B.

Mantovani, A.

Peri, S.

Friess, H. H.

Post, S.

von Knebel Doeberitz, M.

Schwarzbach, M. H. M.

机构：

[1] Univ Heidelberg, Mannheim Univ Clin, Dept Surg, D-68135 Mannheim, Germany

[2] Univ Heidelberg, Mannheim Univ Clin, Inst Clin Chem, D-68135 Mannheim, Germany

[3] Univ Heidelberg, Mannheim Univ Clin, Dept Pathol, D-68135 Mannheim, Germany

[4] Ist Ric Farmacol Mario Negri, Dept Immunol & Cell Biol, I-20157 Milan, Italy

[5] Univ Heidelberg, Dept Surg, D-69120 Heidelberg, Germany

[6] Univ Heidelberg, Dept Pathol, Dept Mol Pathol, D-69120 Heidelberg, Germany

来源：

EUROPEAN JOURNAL OF CANCER | 2006年 / 42卷 / 15期

关键词：

liposarcoma; pentraxin; FUS/CHOP; fusion transcript; PTX3; PRIMITIVE NEUROECTODERMAL TUMOR; GENE-EXPRESSION; FUSION TRANSCRIPTS; SECRETED PROTEIN; EWINGS-SARCOMA; TLS-CHOP; CELLS; PCR; INDUCTION; ONCOGENE;

D O I：

10.1016/j.ejca.2006.05.035

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A unique feature of human soft tissue liposarcoma is a stable (12;16)(q13;p11) translocation. observed mainly in myxoid and roundcell liposarcomas. This translocation. results in FUS/CHOP fusion transcripts with a corresponding oncogenic protein. We hypothesised that genes downstream of FUS/CHOP might serve as attractive candidates for novel tumour associated antigens. Among a panel of analysed genes, only pentraxin related gene (PTX3) demonstrated high expression in liposarcomas as compared to normal tissues. The analysis of RNA and protein expression demonstrated concordant results. However, the level of RNA and protein overexpression did not correlate in all cases. Finally, PTX3 expression was not related to presence of a FUS/CHOP fusion transcript within the liposarcoma tissues. PTX3 has been associated with adipocyte differentiation and now, additionally, is characterised by a markedly increased expression in human soft tissue liposarcoma. This finding mandates further research efforts to clarify the exact role of PTX3 in liposarcoma oncogenesis. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2639 / 2646

页数：8

共 28 条

[1] Characterization of the long pentraxin PTX3 as a TNFα-induced secreted protein of adipose cells
Abderrahim-Ferkoune, A
Bezy, O
Chiellini, C
Maffei, M
Grimaldi, P
Bonino, F
Moustaid-Moussa, N
Pasqualini, F
Mantovani, A
Ailhaud, G
Amri, EZ
[J]. JOURNAL OF LIPID RESEARCH, 2003, 44 (05) : 994 - 1000
[2] A comparison of selected mRNA and protein abundances in human liver
Anderson, L
Seilhamer, J
[J]. ELECTROPHORESIS, 1997, 18 (3-4) : 533 - 537
[3] CHOP (GADD153) AND ITS ONCOGENIC VARIANT, TLS-CHOP, HAVE OPPOSING EFFECTS ON THE INDUCTION OF G(1)/S ARREST
BARONE, MV
CROZAT, A
TABAEE, A
PHILIPSON, L
RON, D
[J]. GENES & DEVELOPMENT, 1994, 8 (04) : 453 - 464
[4] Characterization of the promoter for the human long pentraxin PTX3 - Role of NF-kappa B in tumor necrosis factor-alpha and interleukin-1 beta regulation
Basile, A
Sica, A
dAniello, E
Breviario, F
Garrido, G
Castellano, M
Mantovani, A
Introna, M
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (13) : 8172 - 8178
[5] Quantitative cytokine gene expression in human tonsils at excision and during histoculture assessed by standardized and calibrated real-time PCR and novel data processing
Bonanomi, A
Kojic, D
Giger, B
Rickenbach, Z
Jean-Richard-Dit-Bressel, L
Berger, C
Niggli, FK
Nadal, D
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 283 (1-2) : 27 - 43
[6] BREVIARIO F, 1992, J BIOL CHEM, V267, P22190
[7] MOLECULAR EVENTS IN ADIPOCYTE DEVELOPMENT
BUTTERWITH, SC
[J]. PHARMACOLOGY & THERAPEUTICS, 1994, 61 (03) : 399 - 411
[8] Seminars in medicine of the Beth Israel Hospital, Boston: The RET proto-oncogene in multiple endocrine neoplasia type 2 and Hirschsprung's disease
Eng, C
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1996, 335 (13) : 943 - 951
[9] Fletcher ChristopherDM., 2002, PATHOLOGY GENETICS T, V4
[10] Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility
Garlanda, C
Bottazzi, B
Bastone, A
Mantovani, A
[J]. ANNUAL REVIEW OF IMMUNOLOGY, 2005, 23 : 337 - 366

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