Changes in onion root development induced by the inhibition of peptidyl-prolyl hydroxylase and influence of the ascorbate system on cell division and elongation

被引:93
作者
De Tullio, MC
Paciolla, C
Dalla Veechia, F
Rascio, N
D'Emerico, S
De Gara, L
Liso, R
Arrigoni, O [1 ]
机构
[1] Univ Bari, Ist Bot, Via E Orabona 4, I-70125 Bari, Italy
[2] Univ Padua, Dipartimento Biol, I-35121 Padua, Italy
关键词
Allium (root development); ascorbate; cell division; cell expansion; hydroxyproline-containing protein; root development;
D O I
10.1007/s004250050745
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Post-translational hydroxylation of peptide-bound proline residues, catalyzed by peptidyl-prolyl-4 hydroxylase IEC 1.14.11.2) using ascorbate as cosubstrate, is a key event in the maturation of a number of cell wall-associated hydroxyproline-rich glycoproteins (HRGPs), including extensins and arabinogalactan-proteins, which are involved in the processes of wall stiffening, signalling and cell proliferation. Allium cepa L. roots treated with 3,4-DL-dehydroproline (DP), a specific inhibitor of peptidyl-prolyl hydroxylase, showed a 56% decrease in the hydroxyproline content of HRGP. Administration of DP strongly affected the organization of specialized zones of root development, with a marked reduction of the post-mitotic isodiametric growth zone, early extension of cells leaving the meristematic zone and a huge increase in cell size. Electron-microscopy analysis showed dramatic alterations both to the organization of newly formed cell walls and to the adhesion of the plasma membranes to the cell walls. Moreover, DP administration inhibited cell cycle progression. Root tips grown in the presence of DP also showed an increase both in ascorbate content (+ 53%) and ascorbate-specific peroxidase activity in the cytosol (+ 72%), and a decrease in extracellular "secretory" peroxidase activity (-73%). The possible interaction between HRGPs and the ascorbate system in the regulation of both cell division and extension is discussed.
引用
收藏
页码:424 / 434
页数:11
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