Web-based primer design for single nucleotide polymorphism analysis

被引：500

作者：

Neff, MM ^{[1
]}

Turk, E ^{[1
]}

Kalishman, M ^{[1
]}

机构：

[1] Washington Univ, Dept Biol, St Louis, MO 63130 USA

来源：

TRENDS IN GENETICS | 2002年 / 18卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0168-9525(02)02820-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The detection of single nucleotide polymorphisms by PCR is necessary for many types of genetic analysis, from mapping genomes to tracking specific mutations. This technique is most commonly used when polymorphisms alter restriction endonuclease recognition sites. Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to the polymorphism being analyzed.

引用

页码：613 / 615

页数：3

共 8 条

[1]

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[2] The essence of SNPs [J].