Isolation and characterization by electrospray-ionization mass spectrometry and high-performance anion-exchange chromatography of oligosaccharides derived from hyaluronic acid by hyaluronate lyase digestion: Observation of some heretofore unobserved oligosaccharides that contain an odd number of units

被引：50

作者：

Price, KN

Tuinman, A

Baker, DC

Chisena, C

Cysyk, RL

机构：

[1] UNIV TENNESSEE,DEPT CHEM,KNOXVILLE,TN 37996

[2] NCI,MED CHEM LAB,BETHESDA,MD 20892

来源：

CARBOHYDRATE RESEARCH | 1997年 / 303卷 / 03期

基金：

美国国家科学基金会;

关键词：

hyaluronan; hyaluronic acid; electrospray-ionization mass spectrometry; high-performance anion-exchange chromatography (HPAEC); pulsed amperometric detection (PAD); glycosaminoglycans;

D O I：

10.1016/S0008-6215(97)00171-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hyaluronic acid was degraded with hyaluronate lyase (E.C. 4.2.2.1, from Streptomyces hyalurolyticus), and the resulting oligosaccharides up to dp 16 were characterized by electrospray-ionization mass spectrometry (ESIMS) and high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). In accordance with the known regiospecificity of the enzyme, the products included even-numbered oligosaccharides of structure beta-D-4en-thrHexpA-(1 --> 3)-[beta-D-GlcpNAc-(1 --> 4)-beta-D-GlcpA](n)-(1 --> 3)-D-GlcpNAc. Minor amounts of novel and unexpected odd-numbered oligomers, having the structure beta-D-4en-thrHexpA-(1 --> 3)-[beta-D-GlcpNAc-(1 --> 4)-D-GlcpA](n), were also isolated and characterized. This study, in addition to others beginning to appear in the literature, demonstrates the usefulness of ESIMS and HPAEC-PAD in the analysis and characterization of anionic glycosaminoglycan-type oligosaccharides. (C) 1997 Elsevier Science Ltd.

引用

页码：303 / 311

页数：9

共 46 条

[1] Evaluation of antitumor activities of hyaluronate binding antitumor drugs: Synthesis, characterization and antitumor activity [J].