Exploring the binding profiles of ConA, boronic acid and WGA by MALDI-TOF/TOF MS and magnetic particles

被引：81

作者：

Sparbier, Katrin ^{[1
]}

Wenzel, Thomas ^{[1
]}

Kostrzewa, Markus ^{[1
]}

机构：

[1] Bruker Daltonik GMBH, D-04318 Leipzig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 840卷 / 01期

关键词：

serum glycoproteins; MALDI-TOF/TOF MS; magnetic particles;

D O I：

10.1016/j.jchromb.2006.06.028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Concanavalin A, boronic acid and Wheat germ agglutinin functionalized magnetic micro-particles were developed to enrich glycosylated peptides and proteins. The bead functionalities were validated according to their specificity by analyses of model proteins. Validated beads were employed for the enrichment of glycosylated human serum proteins. Eluted glycoproteins were digested by trypsin and the resulting peptides were purified by magnetic MB-HIC C8 beads. Each fraction was analyzed by MALDI-TOF MS and single peaks were subjected to MALDI-TOF/TOF MS with the objective to identify the respective proteins by database search. Search results revealed overlapping profiles of known serum glycoproteins. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：29 / 36

页数：8

共 13 条

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