Characterization of a trimeric MPER containing HIV-1 gp41 antigen

被引：30

作者：

Hinz, Andreas ^{[1
]}

Schoehn, Guy ^{[1
,2
]}

Quendler, Heribert ^{[3
]}

Hulsik, David Lutje ^{[1
]}

Stiegler, Gabi ^{[3
]}

Katinger, Hermann ^{[3
]}

Seaman, Michael S. ^{[5
]}

Montefiori, David ^{[4
]}

Weissenhorn, Winfried ^{[1
]}

机构：

[1] UJF EMBL CNRS, UMI 3265, Unit Virus Host Cell Interact, F-38042 Grenoble 9, France

[2] CEA CNRS UJF, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble 1, France

[3] Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Inst Appl Microbiol, A-1190 Vienna, Austria

[4] Duke Univ, Med Ctr, Lab AIDS Res & Dev, Dept Surg, Durham, NC 27705 USA

[5] Beth Israel Deaconess Med Ctr, Div Viral Pathogenesis, Boston, MA 02215 USA

来源：

VIROLOGY | 2009年 / 390卷 / 02期

关键词：

HIV-1; gp41; mAb; 2F5; 4E10; Membrane fusion; HUMAN-IMMUNODEFICIENCY-VIRUS; PROXIMAL EXTERNAL REGION; HUMAN MONOCLONAL-ANTIBODY; BROADLY NEUTRALIZING ANTIBODIES; STRUCTURAL BASIS; TYPE-1; ANTIBODY; MEMBRANE-FUSION; TRANSMEMBRANE GLYCOPROTEIN; VACCINE DESIGN; ANTI-GP41; 2F5;

D O I：

10.1016/j.virol.2009.05.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The membrane-proximal external region (MPER) of gp41 is considered as a prime target for the induction of neutralizing antibodies, since it contains the epitopes for three broadly neutralizing antibodies (2F5, 4E10 and Z13). Here we present a novel gp41 construct (HA-gp41) comprising gp41 HR2 and MPER fused to two triple-stranded coiled-coil domains at both ends. HA-gp41 is trimeric, has a high helical content in solution and forms rod-like structures as revealed by negative staining electron microscopy. Immunization of rabbits with HA-gp41 induced antibodies directed against MPER, which failed to exert significant neutralization capacity against envelopes from primary isolates. Thus trimerisation of MPER regions does not suffice to induce a potent neutralizing antibody response specific for conserved regions within gp41. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：221 / 227

页数：7

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