Temperature-dependent phosphorylation state of the H5R protein synthesised at the early stage of infection in cells infected with vaccinia virus ts mutants of the B1R and F10L protein kinases

被引:10
作者
Beaud, G
Beaud, R
机构
[1] Univ Paris 06, CNRS, Inst Jacques Monod, F-75251 Paris 05, France
[2] Univ Paris 07, Paris, France
关键词
poxviridae; vaccinia virus; ts mutants; protein kinase;
D O I
10.1159/000025025
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes, A temperature-dependent increase of underphosphorylated H5R protein (pl 6.8) was demonstrated in the case of the B1R, but not of the F10L gene, The temperature-dependent cytoplasmic location of underphosphorylated H5R protein after infection with the ts mutants of the B1R gene was the consequence of the associated viral DNA replication block, These results show that the B1R protein kinase controls the phosphorylation state of the H5R protein synthesised at the early stage in vaccinia-virus-infected cells, Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:67 / 70
页数:4
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