Vascular endothelial growth factor-loaded injectable hydrogel enhances plasticity in the injured spinal cord

被引：58

作者：

des Rieux, Anne ^{[1
]}

De Berdt, Pauline ^{[1
]}

Ansorena, Eduardo ^{[1
]}

Ucakar, Bernard ^{[1
]}

Jacobs, Damien ^{[1
]}

Schakman, Olivier ^{[2
]}

Audouard, Emilie ^{[3
]}

Bouzin, Caroline ^{[4
]}

Auhl, Dietmar ^{[5
]}

Simon-Yarza, Teresa ^{[6
]}

Feron, Olivier ^{[4
]}

Blanco-Prieto, Maria J. ^{[6
]}

Carmeliet, Peter ^{[7
]}

Bailly, Christian ^{[5
]}

Clotman, Frederic ^{[3
]}

Preat, Veronique ^{[1
]}

机构：

[1] Catholic Univ Louvain, Louvain Drug Res Inst, Pharmaceut & Drug Delivery Unit, B-1200 Brussels, Belgium

[2] Catholic Univ Louvain, Inst Neurosci, Lab Cell Physiol, B-1200 Brussels, Belgium

[3] Catholic Univ Louvain, Inst Neurosci, Lab Neural Differentiat, B-1200 Brussels, Belgium

[4] Catholic Univ Louvain, Inst Rech Expt & Clin, B-1200 Brussels, Belgium

[5] Catholic Univ Louvain, Bio & Soft Matter Unit, Inst Condensed Matter & Nanosci, B-1148 Louvain La Neuve, Belgium

[6] Univ Navarra, Sch Pharm, Pharm & Pharmaceut Technol Dept, E-31080 Pamplona, Spain

[7] VIB Univ Leuven, VRC, Lab Angiogenesis & Neurovasc Link, Dept Oncol, Leuven, Belgium

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2014年 / 102卷 / 07期

关键词：

injectable hydrogel; VEGF delivery; nanoparticles; microparticles; alginate; spinal cord; MARROW STROMAL CELLS; ALGINATE HYDROGELS; NERVOUS-SYSTEM; VEGF; DELIVERY; ANGIOGENESIS; RECOVERY; SURVIVAL; RELEASE; BRIDGES;

D O I：

10.1002/jbm.a.34915

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

We hypothesized that vascular endothelial growth factor (VEGF)-containing hydrogels that gelify in situ after injection into a traumatized spinal cord, could stimulate spinal cord regeneration. Injectable hydrogels composed of 0.5% Pronova UPMVG MVG alginate, supplemented or not with fibrinogen, were used. The addition of fibrinogen to alginate had no effect on cell proliferation in vitro but supported neurite growth ex vivo. When injected into a rat spinal cord in a hemisection model, alginate supplemented with fibrinogen was well tolerated. The release of VEGF that was incorporated into the hydrogel was influenced by the VEGF formulation [encapsulated in microspheres or in nanoparticles or in solution (free)]. A combination of free VEGF and VEGF-loaded nanoparticles was mixed with alginate:fibrinogen and injected into the lesion of the spinal cord. Four weeks post injection, angiogenesis and neurite growth were increased compared to hydrogel alone. The local delivery of VEGF by injectable alginate:fibrinogen-based hydrogel induced some plasticity in the injured spinal cord involving fiber growth into the lesion site. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2345-2355, 2014.

引用

页码：2345 / 2355

页数：11

共 52 条

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