Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control

被引：181

作者：

Hasan, MT ^{[1
]}

Friedrich, RW

Euler, T

Larkum, ME

Giese, G

Both, M

Duebel, J

Waters, J

Bujard, H

Griesbeck, O

Tsien, RY

Nagai, T

Miyawaki, A

Denk, W

机构：

[1] Max Planck Inst Med Res, Heidelberg, Germany

[2] Heidelberg Univ, Zentrum Mol Biol, Heidelberg, Germany

[3] Univ Calif San Diego, Dept Pharmacol, San Diego, CA USA

[4] Univ Calif San Diego, Howard Hughes Med Inst, San Diego, CA USA

[5] RIKEN, Inst Brain Res, Saitama, Japan

来源：

PLOS BIOLOGY | 2004年 / 2卷 / 06期

关键词：

D O I：

10.1371/journal.pbio.0020163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.

引用

页码：763 / 775

页数：13

共 72 条

[1] Circular permutation and receptor insertion within green fluorescent proteins [J].