Structure of the 70S ribosome complexed with mRNA and tRNA

被引：1037

作者：

Selmer, Maria ^{[1
]}

Dunham, Christine M. ^{[1
]}

Murphy, Frank V. ^{[1
]}

Weixlbaumer, Albert ^{[1
]}

Petry, Sabine ^{[1
]}

Kelley, Ann C. ^{[1
]}

Weir, John R. ^{[1
]}

Ramakrishnan, V. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

SCIENCE | 2006年 / 313卷 / 5795期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1126/science.1131127

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA ( mRNA) and transfer RNA ( tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

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页码：1935 / 1942

页数：8

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