Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex

被引：31

作者：

Ajuh, PM

Browne, GJ

Hawkes, NA

Cohen, PTW

Roberts, SGE

Lamond, AI

机构：

[1] Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland

[2] Univ Dundee, Dept Biochem, MRC, Prot Phosphorylat Unit, Dundee DD1 5EH, Scotland

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/28.3.678

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have screened a human cDNA expression library with a digoxygenin-labelled protein phosphatase 1 (PP1) probe to identify novel PP1 interacting proteins. Eleven cDNA clones were isolated, which included genes encoding two previously characterised and six novel PP1 binding proteins. Three of the cDNAs encoded a protein called host cell factor (HCF), which is an essential component of the cellular complex required for the transcription of the herpes simplex virus (HSV) immediate-early (IE) genes. We demonstrate that HCF and PP1 exist as a complex in nuclear extracts and that this complex is distinct from the form of HCF that associates with HSV VP16, The data suggest novel roles for HCF and PP1, which may be relevant to their functions in transcription and cell cycle progression.

引用

收藏

页码：678 / 686

页数：9

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