Targeted inoculation of Medicago truncatula in vitro root cultures reveals MtENOD11 expression during early stages of infection by arbuscular mycorrhizal fungi

被引:83
作者
Chabaud, M
Venard, C
Defaux-Petras, A
Bécard, G
Barker, DG
机构
[1] INRA, CNRS, UMR 215, Lab Biol Mol Relat Plantes Microorganismes, F-31326 Castanet Tolosan, France
[2] Univ Toulouse 3, CNRS, UMR 5546, F-31326 Castanet Tolosan, France
关键词
Agrobacterium rhizogenes; appressorium; arbuscular mycorrhiza; early nodulin; Gigaspora; Medicago truncatula; Myc(-) mutant; root symbiosis;
D O I
10.1046/j.1469-8137.2002.00508.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An in vitro targeted inoculation technique has been developed for studying the earliest stages of arbuscular endomycorrhizal (AM) infection of Medicago truncatula roots, and in particular the spatio-temporal expression of the early nodulin gene MtENOD11. Agrobacterium rhizogenes transformed root explants were derived either from Myc(+) M. truncatula or from the infection-defective Myc(-) mutant TR26 (dmi2-2), both expressing the pMtENOD11-gusA fusion. The normal positive geotropism of these roots, coupled with the negative geotropism of Gigaspora germ tubes allowed oriented growth of the two symbiotic partners, facilitating the identification of initial fungal/root contacts. Early infection events at the stage of appressoria and/or internal hyphae could be observed for over 50% of the inoculated explants, revealing that MtENOD11 is expressed transiently in both epidermal and cortical cells at sites of hyphal penetration in Myc(+) roots, but not in epidermal cells in contact with appressoria in Myc(-) roots. We propose that a direct link exists between MtENOD11 gene expression and cellular events required for fungal penetration, thereby extending analogies between rhizobial and AAA host root infection processes.
引用
收藏
页码:265 / 273
页数:9
相关论文
共 42 条
[1]   Bayesian models for keyhole plan recognition in an adventure game [J].
Albrecht, DW ;
Zukerman, I ;
Nicholson, AE .
USER MODELING AND USER-ADAPTED INTERACTION, 1998, 8 (1-2) :5-47
[2]  
ANE JM, 2002, IN PRESS MOL PLANT M, V15
[3]   PISUM-SATIVUM MUTANTS INSENSITIVE TO NODULATION ARE ALSO INSENSITIVE TO INVASION IN-VITRO BY THE MYCORRHIZAL FUNGUS, GIGASPORA-MARGARITA [J].
BALAJI, B ;
BA, AM ;
LARUE, TA ;
TEPFER, D ;
PICHE, Y .
PLANT SCIENCE, 1994, 102 (02) :195-203
[4]   PHYSIOLOGICAL FACTORS DETERMINING VESICULAR-ARBUSCULAR MYCORRHIZAL FORMATION IN HOST AND NONHOST RI T-DNA TRANSFORMED ROOTS [J].
BECARD, G ;
PICHE, Y .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1990, 68 (06) :1260-1264
[5]  
BECARD G, 1992, APPL ENVIRON MICROB, V58, P821
[6]   EARLY EVENTS OF VESICULAR ARBUSCULAR MYCORRHIZA FORMATION ON RI T-DNA TRANSFORMED ROOTS [J].
BECARD, G ;
FORTIN, JA .
NEW PHYTOLOGIST, 1988, 108 (02) :211-218
[7]   Induction of Ltp (lipid transfer protein) and Pal (phenylalanine ammonia-lyase) gene expression in rice roots colonized by the arbuscular mycorrhizal fungus Glomus mosseae [J].
Blilou, I ;
Ocampo, JA ;
García-Garrido, JM .
JOURNAL OF EXPERIMENTAL BOTANY, 2000, 51 (353) :1969-1977
[8]   Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations [J].
Boisson-Dernier, A ;
Chabaud, M ;
Garcia, F ;
Bécard, G ;
Rosenberg, C ;
Barker, DG .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2001, 14 (06) :695-700
[9]   The Lotus japonicus LjS']jSym4 gene is required for the successful symbiotic infection of root epidermal cells [J].
Bonfante, P ;
Genre, A ;
Faccio, A ;
Martini, I ;
Schauser, L ;
Stougaard, J ;
Webb, J ;
Parniske, M .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (10) :1109-1120
[10]   INTERACTIONS BETWEEN 3 ALFALFA NODULATION GENOTYPES AND 2 GLOMUS SPECIES [J].
BRADBURY, SM ;
PETERSON, RL ;
BOWLEY, SR .
NEW PHYTOLOGIST, 1991, 119 (01) :115-120