A simple method for making reproducible fibre loops for protein cryocrystallography

被引:19
作者
Sauer, UH [1 ]
Ceska, TA [1 ]
机构
[1] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY
关键词
D O I
10.1107/S0021889896008047
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A practical, step-by-step description of the construction of loops for protein cryocrystallography using pulled-out microinjection capillaries is described. The advantages of this method are the high reproducibility of the loops, the ease of adjustment of the loop diameter and the formation of thin films owing to the narrow base of the loop. During assembly, the loop does not make contact with either glue or cement.
引用
收藏
页码:71 / 72
页数:2
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