Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice

被引:91
作者
Xu, Huawei [1 ]
Zhang, Jianjun [1 ,2 ]
Zeng, Jiwu [1 ]
Jiang, Linrong [1 ]
Liu, Ee [1 ,2 ]
Peng, Changlian [3 ]
He, Zhenghui [4 ]
Peng, Xinxiang [1 ,2 ]
机构
[1] S China Agr Univ, Coll Life Sci, Lab Mol Plant Physiol, Guangzhou 510642, Guangdong, Peoples R China
[2] S China Agr Univ, Key Lab Plant Funct Genom & Biotechnol, Educ Dept Guangdong Prov, Guangzhou 510642, Guangdong, Peoples R China
[3] S China Normal Univ, Coll Life Sci, Guangzhou 510640, Guangdong, Peoples R China
[4] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA
基金
中国国家自然科学基金;
关键词
Glycolate oxidase (GLO); glyoxylate cycle; inducible antisense; photorespiration; photosynthesis; rice; LIQUID-CHROMATOGRAPHIC DETERMINATION; GLYOXYLATE AMINOTRANSFERASE ACTIVITY; RIBULOSE BISPHOSPHATE CARBOXYLASE; ARABIDOPSIS MUTANTS LACKING; GLYCINE DECARBOXYLASE; GLUTAMATE SYNTHASE; PHOTO-RESPIRATION; REDUCED ACTIVITIES; CO2; ASSIMILATION; RUBP CARBOXYLASE;
D O I
10.1093/jxb/erp056
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Photorespiration is one of the most intensively studied topics in plant biology. While a number of mutants deficient in photorespiratory enzymes have been identified and characterized for their physiological functions, efforts on glycolate oxidase (GLO; EC 1.1.3.15) have not been so successful. This is a report about the generation of transgenic rice (Oryza sativa L.) plants carrying a GLO antisense gene driven by an estradiol-inducible promoter, which allowed for controllable suppressions of GLO and its detailed functional analyses. The GLO-suppressed plants showed typical photorespiration-deficient phenotypes. More intriguingly, it was found that a positive and linear correlation existed between GLO activities and the net photosynthetic rates (P-N), and photoinhibition subsequently occurred once P-N reduction surpassed 60%, indicating GLO can exert a strong regulation over photosynthesis. Various expression analyses identified that Rubisco activase was transcriptionally suppressed in the GLO-suppressed plants, consistent with the decreased Rubisco activation states. While the substrate glycolate accumulated substantially, few changes were observed for the product glyoxylate, and for some other downstream metabolites or genes as well in the transgenic plants. Further analyses revealed that isocitrate lyase and malate synthase, two key enzymes in the glyoxylate cycle, were highly up-regulated under GLO deficiency. Taken together, the results suggest that GLO is a typical photorespiratory enzyme and that it can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase, and that the glyoxylate cycle may be partially activated to compensate for the photorespiratory glyoxylate when GLO is suppressed in rice.
引用
收藏
页码:1799 / 1809
页数:11
相关论文
共 56 条
[1]  
[Anonymous], 1989, Molecular Cloning: A Laboratory Manual
[2]   Production and scavenging of reactive oxygen species in chloroplasts and their functions [J].
Asada, Kozi .
PLANT PHYSIOLOGY, 2006, 141 (02) :391-396
[3]   A glycolate dehydrogenase in the mitochondria of Arabidopsis thaliana [J].
Bari, R ;
Kebeish, R ;
Kalamajka, R ;
Rademacher, T ;
Peterhänsel, C .
JOURNAL OF EXPERIMENTAL BOTANY, 2004, 55 (397) :623-630
[4]   Genetic manipulation of glycine decarboxylation [J].
Bauwe, H ;
Kolukisaoglu, Ü .
JOURNAL OF EXPERIMENTAL BOTANY, 2003, 54 (387) :1523-1535
[5]   THE VALUE OF MUTANTS UNABLE TO CARRY OUT PHOTORESPIRATION [J].
BLACKWELL, RD ;
MURRAY, AJS ;
LEA, PJ ;
KENDALL, AC ;
HALL, NP ;
TURNER, JC ;
WALLSGROVE, RM .
PHOTOSYNTHESIS RESEARCH, 1988, 16 (1-2) :155-176
[6]   D-GLYCERATE 3-KINASE, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family [J].
Boldt, R ;
Edner, C ;
Kolukisaoglu, Ü ;
Hagemann, M ;
Weckwerth, W ;
Wienkoop, S ;
Morgenthal, K ;
Bauwe, H .
PLANT CELL, 2005, 17 (08) :2413-2420
[7]   PHOTORESPIRATION-INDUCED REDUCTION OF RIBULOSE BISPHOSPHATE CARBOXYLASE ACTIVATION LEVEL [J].
CHASTAIN, CJ ;
OGREN, WL .
PLANT PHYSIOLOGY, 1985, 77 (04) :851-856
[8]  
COMAI L, 1989, PLANT CELL, V1, P292
[9]   Lipid utilization, gluconeogenesis, and seedling growth in Arabidopsis mutants lacking the glyoxylate cycle enzyme malate synthase [J].
Cornah, JE ;
Germain, V ;
Ward, JL ;
Beale, MH ;
Smith, SM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (41) :42916-42923
[10]  
Dumbroff Erwin B., 1993, P207