Presence of translation elongation factor-1A (eEF1A) in the excitatory postsynaptic density of rat cerebral cortex

被引：11

作者：

Cho, SJ

Jung, JS

Ko, BH

Jin, I

Moon, IS

机构：

[1] Dongguk Univ, Coll Med, Dept Anat, Seoul 780714, South Korea

[2] Dongguk Univ, Coll Med, Inst Med, Seoul 780714, South Korea

[3] Kyungpook Natl Univ, Coll Nat Sci, Dept Microbiol, Taegu 702701, South Korea

来源：

NEUROSCIENCE LETTERS | 2004年 / 366卷 / 01期

关键词：

eEFIA; immunoblot; immunocytochemistry; PSD; synapse;

D O I：

10.1016/j.neulet.2004.05.036

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The postsynaptic density (PSD) is a proteinaceous cellular structure that is specialized for postsynaptic signal transduction. Here, we show that eukaryotic translation factor-1A (eEF1A; formerly known as eEF-1alpha) is associated with the excitatory PSD in rat forebrain. Immunoblot analysis showed that eEF1A in the PSD fraction is enriched over homogenate. Salt (1.0 M NaCl), but not non-ionic detergents such as Triton X-100 (1.0%) and n-octyl glucoside (1.0%), could dissociate eEF1A from the PSD core. In cultured cortical neurons, eEF1A was colocalized with postsynaptic markers (PSD95 and SynGAPalpha), but not with a presynaptic marker (synaptophysin). These results indicate that eEF1A is present in the PSD of the excitatory synapses. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：29 / 33

页数：5

共 24 条

[1]

Apperson ML, 1996, J NEUROSCI, V16, P6839

[2] Association of GAP-43 with detergent-resistant membranes requires two palmitoylated cysteine residues [J].