Giant liposomes: from membrane dynamics to cell morphogenesis

被引：81

作者：

Hotani, H ^{[1
]}

Nomura, F ^{[1
]}

Suzuki, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Dept Mol Biol, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE | 1999年 / 4卷 / 05期

关键词：

giant liposome; shape change; membrane; cytoskeleton; artificial cell; fusion;

D O I：

10.1016/S1359-0294(99)90021-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Although a lipid bilayer is only several nanometers in thickness, giant liposomes (GLs) make it possible to observe the dynamic features of individual membrane vesicles in real time with optical microscopy. Recent progress in GL preparation methods allows one to make reproducible image analyses of essential characteristics of membrane vesicles including fusion, division and poration processes. As a model of living cells, morphological changes of GLs that encapsulate cytoskeletal filaments such as microtubules or F-actin can be investigated directly. Applications of GLs based on the advantages of their large size is described using patch clamps, injection, mechanical transducer and so on. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：358 / 368

页数：11

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