MALDI Profiling and Applications in Medicine

被引：6

作者：

Dudley, Ed ^{[1
]}

机构：

[1] Swansea Univ, Coll Med, Inst Mass Spectrometry, Swansea, W Glam, Wales

来源：

ADVANCEMENTS OF MASS SPECTROMETRY IN BIOMEDICAL RESEARCH | 2014年 / 806卷

关键词：

ASSISTED-LASER-DESORPTION/IONIZATION; FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; SINGLE NUCLEOTIDE POLYMORPHISMS; MAGNETIC BEAD SEPARATION; SERUM-PROTEIN BIOMARKERS; POSITIVE BLOOD CULTURES; RAPID IDENTIFICATION; PHOSPHORYLATED PEPTIDES; STAPHYLOCOCCUS-AUREUS;

D O I：

10.1007/978-3-319-06068-2_2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry allows for the rapid profiling of different biomolecular species from both biofluids and tissues. Whilst originally focussed upon the analysis of intact proteins and peptides, MALDI mass spectrometry has found further applications in lipidomic analysis, genotyping, micro-organism identification, biomarker discovery and metabolomics. The combining of multiple profiles data from differing locations across a sample, furthermore, allows for spatial distribution of biomolecules to be established utilising imaging MALDI analysis. This chapter discusses the MALDI process, its usual applications in the field of protein identification via peptide mass fingerprinting before focusing upon advances in the application of the profiling potential of MALDI mass spectrometry and its various applications in biomedicine.

引用

页码：33 / 58

页数：26

共 100 条

[1] Protein and peptide profiling as a tool for biomarker discovery in depression [J].

AlAwam, Khaled ;

Dudley, Ed ;

Donev, Rossen ;

Thome, Johannes .

ELECTROPHORESIS, 2012, 33 (24) :3830-3834

[2] Distinct cerebrospinal fluid amyloid-beta peptide signatures in cognitive decline associated with Alzheimer's disease and schizophrenia [J].

Albertini, Valentina ;

Benussi, Luisa ;

Paterlini, Anna ;

Glionna, Michela ;

Prestia, Annapaola ;

Bocchio-Chiavetto, Luisella ;

Amicucci, Giovanni ;

Galluzzi, Samantha ;

Adorni, Andrea ;

Geroldi, Cristina ;

Binetti, Giuliano ;

Frisoni, Giovanni B. ;

Ghidoni, Roberta .

ELECTROPHORESIS, 2012, 33 (24) :3738-3744

[3]

An Yanming, 2013, Methods Mol Biol, V1023, P13, DOI 10.1007/978-1-4614-7209-4_2

[4]

[Anonymous], OPHTHALMIC GENET

[5]

[Anonymous], J MICROBIOL METHODS

[6]

[Anonymous], ANAL BIOCH

[7]

[Anonymous], GENE

[8]

[Anonymous], DIAGN MICROBIOL INFE

[9] Impact of sample preparation in peptide/protein profiling in human serum by MALDI-TOF mass spectrometry [J].

Aresta, Antonella ;

Calvano, Cosima D. ;

Palmisano, Francesco ;

Zambonin, Carlo G. ;

Monaco, Alessandro ;

Tornmasi, Stefania ;

Pilato, Brunella ;

Paradiso, Angelo .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2008, 46 (01) :157-164

[10] Optimizing protein recovery yield from serum samples treated with beads technology [J].

Bellei, Elisa ;

Monari, Emanuela ;

Bergamini, Stefania ;

Ozben, Tomris ;

Tomasi, Aldo .

ELECTROPHORESIS, 2011, 32 (12) :1414-1421

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