Probing solvent fractionation in electrospray droplets with laser-induced fluorescence of a solvatochromic dye

被引：60

作者：

Zhou, SL ^{[1
]}

Cook, KD ^{[1
]}

机构：

[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 05期

关键词：

D O I：

10.1021/ac990912n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Laser-induced fluorescence spectroscopy is used to profile soh ent fractionation in an electrospray plume containing Nile Red, a solvatochromic dye (i.e., a dye for which spectral features are sensitive to solvent polarity). The results confirm gradual enrichment of the less volatile component in spray droplets as a result of solvent evaporation. Changes in solvent composition are evident in both axial and lateral profiles. The influences of capillary voltage, nebulizing gas, an electrolyte modifier, and now rate on the extent of solvent fractionation are assessed. The results suggest that, in addition to volatility of the solvents, major factors affecting solvent fractionation include initial droplet size, droplet velocity, and the cooling effect of the nebulizing gas. Isolating contributions from these factors will require complementary data from other experiments.

引用

页码：963 / 969

页数：7

共 42 条

[1] DOES THE OBSERVATION OF NONCOVALENT COMPLEXES BETWEEN BIOMOLECULES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY NECESSARILY REFLECT SPECIFIC SOLUTION INTERACTIONS [J].