Identification of a novel mouse membrane-bound family 1 glycosidase-like protein, which carries an atypical active site structure

被引：89

作者：

Ito, S

Fujimori, T

Hayashizaki, Y

Nabeshima, Y

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Pathol & Tumor Biol, Sakyo Ku, Kyoto 6068501, Japan

[2] RIKEN Yokohama Inst, RIKEN Genom Sci Ctr, Lab Genome Explorat Res Grp, Tsurumi Ku, Kanagawa 2300045, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2002年 / 1576卷 / 03期

关键词：

klotho; beta klotho; Klotho family; LPH; family; 1; glycosidase; glutamic acid;

D O I：

10.1016/S0167-4781(02)00281-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a novel mouse gene klph (Klotho-LPH related protein; where LPH stands for lactase-phlorizin hydrolase) that encodes a novel mammalian family I glycosidase-like protein. KLPH was a putative type I membrane protein that consists of N-terminal signal sequence, glycosidase domain, transmembrane region and short cytoplasmic tail. Despite its overall structural similarity to other family I glycosidases, the glutamic acid for the acid-base catalyst was not conserved in this protein. klph mRNA was predominantly expressed in the kidney and skin. Epitope-tagged KLPH was localized to the perinuclear tubular network structure of the endoplasmic reticulum in cultured cells. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：341 / 345

页数：5

共 24 条

[1] The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidase [J].