Mutations at codon 184 in simian immunodeficiency virus reverse transcriptase confer resistance to the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine

被引：15

作者：

Cherry, E

Slater, M

Salomon, H

Rud, E

Wainberg, MA

机构：

[1] MCGILL UNIV,JEWISH GEN HOSP,LADY DAVIS INST MED RES,AIDS CTR,MONTREAL,PQ H3T 1E2,CANADA

[2] LAB CTR DIS CONTROL,OTTAWA,ON K1A 0L2,CANADA

[3] UNIV BUENOS AIRES,SCH MED,DEPT MICROBIOL,RA-1121 BUENOS AIRES,DF,ARGENTINA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1997年 / 41卷 / 12期

关键词：

D O I：

10.1128/AAC.41.12.2763

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Variants of simian immunodeficiency virus (SIV) that display greater than 2,000-fold resistance to the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine (3TC) were generated through in vitro passage and drug selection. The polymerase regions of several of these resistant viruses were sequenced and were found to share either of two codon alterations at site 184 in reverse transcriptase (ATG to ATA [methionine to isoleucine] and ATG to GTA [methionine to valine]). The biological relevance of these substitutions for 3TC was confirmed by site-directed mutagenesis with the SIVmac239 infectious recombinant clone of SIV.

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页码：2763 / 2765

页数：3

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