Comparing coherent and spontaneous Raman scattering under biological imaging conditions

被引：49

作者：

Cui, Meng ^{[1
]}

Bachler, Brandon R. ^{[1
]}

Ogilvie, Jennifer P. ^{[1
]}

机构：

[1] Univ Michigan, Dept Phys & Biophys, Ann Arbor, MI 48109 USA

来源：

OPTICS LETTERS | 2009年 / 34卷 / 06期

基金：

美国国家科学基金会;

关键词：

CELLULAR-RESPONSE; LASER-PULSES; MICROSCOPY; SPECTROSCOPY; CARS;

D O I：

10.1364/OL.34.000773

中图分类号：

O43 [光学];

学科分类号：

070207 [光学];

摘要：

We compare imaging based on coherent and spontaneous Raman scattering (SpRS) under conditions relevant for biological imaging. Using a broadband laser source, we perform spectral domain imaging of polystyrene beads using coherent Stokes Raman scattering and SpRS and find comparable signal levels. Short interaction lengths, low molecule number, and low incident power all reduce the advantages available with coherent Raman methods. We present calculations to support our measurements. (C) 2009 Optical Society of America

引用

页码：773 / 775

页数：3

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