First use of two-dimensional polyacrylamide gel electrophoresis to determine phylogenetic relationships

被引：15

作者：

Dopson, M

Baker-Austin, C

Bond, PL ^{[1
]}

机构：

[1] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England

[2] Univ E Anglia, Ctr Ecol Evolut & Conservat, Norwich NR4 7TJ, Norfolk, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 58卷 / 03期

关键词：

2D-PAGE; chemotaxonomy; Ferroplasma; Archaea; acidophile;

D O I：

10.1016/j.mimet.2004.04.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for microbial classification are not always capable of distinguishing between isolates at the species level. We have previously characterised four Ferroplasma isolates that were >98.9% similar at the 16S rDNA level, the isolates showed marked phenotypic differences.. and one isolate was borderline on the 70% species boundary from DNA-DNA similarity data. In this study we have used statistical comparisons of two-dimensional polyacylamide gel electrophoresis gels for classification of closely related isolates. From the protein profile similarities an un-rooted tree was constructed that was congruent with a tree derived from DNA-DNA similarities. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：297 / 302

页数：6

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