The in vitro DNA binding properties of NDP kinase are related to its oligomeric state

被引：18

作者：

Mesnildrey, S ^{[1
]}

Agou, F ^{[1
]}

Veron, M ^{[1
]}

机构：

[1] INST PASTEUR,UNITE REGULAT ENZYMAT ACTIVITIES CELLULAIRES,CNRS,UMR 321,F-75724 PARIS 15,FRANCE

来源：

FEBS LETTERS | 1997年 / 418卷 / 1-2期

关键词：

nucleoside diphosphate kinase; DNA binding protein; dimeric mutant; filter binding assay; development;

D O I：

10.1016/S0014-5793(97)01292-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic and biochemical evidences suggest that the enzymatic activity of NDP kinase is necessary but not sufficient for its biological function, While the human NDPK-B binds specifically single-strand polypyrimidines sequences, the hexameric enzyme from Dictyostelium does not, We demonstrated by electrophoretic mobility shift assay and filter binding assay that a dimeric mutant from Dictyostelium binds to an oligodesoxynucleotide while the wild-type does not, These data suggest that the differences in the DNA binding properties of several eucaryotic NDP kinases might be correlated to the differences in the stability of their hexameric structure. (C) 1997 Federation of European Biochemical Societies.

引用

页码：53 / 57

页数：5

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