Reverse gyrase is not a prerequisite for hyperthermophilic life

被引：89

作者：

Atomi, H

Matsumi, R

Imanaka, T ^{[1
]}

机构：

[1] Kyoto Univ, Dept Synthet Chem & Biol Chem, Grad Sch Engn, Nishikyo Ku, Kyoto 6158510, Japan

[2] Kyoto Univ, Katsura Int Tech Ctr, Nishikyo Ku, Kyoto 6158510, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 14期

关键词：

D O I：

10.1128/JB.186.14.4829-4833.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1 An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90degreesC. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.

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页码：4829 / 4833

页数：5

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