Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins

被引：77

作者：

Rodas, Claudia ^{[1
,2
,3
]}

Iniguez, Volga ^{[3
]}

Qadri, Firdausi ^{[4
]}

Wiklund, Gudrun ^{[1
,2
]}

Svennerholm, Ann-Mari ^{[1
,2
]}

Sjoeling, Asa ^{[1
,2
]}

机构：

[1] Univ Gothenburg, Inst Biomed, Dept Microbiol & Immunol, S-40530 Gothenburg, Sweden

[2] Univ Gothenburg, WHO Collaborating Ctr Res Enterotoxigen Escherich, S-40530 Gothenburg, Sweden

[3] Univ Mayor San Andres, Inst Mol Biol & Biotechnol, Fac Ciencias Puras & Nat, La Paz, Bolivia

[4] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 04期

基金：

瑞典研究理事会;

关键词：

DEVELOPING-COUNTRIES; DISEASE BURDEN; CHILDREN; BANGLADESH; DIARRHEA; INFANTS; PILUS; ETEC;

D O I：

10.1128/JCM.00316-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

引用

页码：1218 / 1220

页数：3

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